本研究室簡介

研究重點(一): 

了解病毒與宿主的免疫反應的相互作用。利用單純性疱疹一型病毒(herpes simplex virus-1, HSV-1)以及動物實驗模式探討周邊組織發炎反應對於HSV-1在皮膚與神經的致病性。

   IL-15異構蛋白是我們的主要研究對象。雖然IL-15是維持自然殺手細胞 (natural killer cells)的發育以及記憶性CD8+ T 細胞的生成與恆定的重要細胞激素，IL-15異構蛋白卻具有調節發炎反應的作用。將表現該異構蛋白ENU 突變鼠的皮膚感染HSV-1後，不僅多種HSV-1病毒誘導的趨化激素與發炎激素的表現量下降，HSV-1在皮膚形成的疱疹傷痂更嚴重而且持續更久，顯示HSV-1容易造成該突變鼠的背根神經感染、提高病毒再次活化的可能。以CD8 tetramer (美國NIH 提供)檢測抗原專一性CD8+ T 細胞，進一步證實ENU 突變鼠產生抗HSV-1 CD8+ T 細胞免疫反應較野生型小鼠下降。利用這項特殊新穎的實驗模式，希望能更進一步了解產生有效的抗病毒CD8 免疫活性的分子機制。

研究重點(二):

台灣地區自從2013年首宗H7N9禽流感境外移入病例後，隨後2015年爆發最大規模的H5N2禽流感流行; 2017年又陸續發生新型H5N8以及H5N6禽流感病毒造成禽場得疫情，帶來糧食安全的重大威脅。這相研究計畫的主要目標是建立雞禽的動物模式，探討新佐劑類病毒顆粒禽流感疫苗的免疫作用機制、鑑別疫苗誘導抗體生成相關的免疫分子標記以及發展評估疫苗性抗體反應的檢測方法，做為未來禽流感疫苗接種的基礎，提高雞禽對於不同高致病性H5 病毒的保護力。希望能提供未來發展減少疫苗失效、降低新病毒演化與預防未來大規模的流行爆發的最佳禽流感疫苗防治策略。

  本實驗室竭誠歡迎有志學習病毒性傳染病免疫學的學習者加入。

1. Yi-Hua Pan, Mei-Ying Liao, Yu-Wen Chien, Tzong-Shiann Ho, Hui-Ying Ko,   Chin-Rur Yang, Shu-Fen Chang, Chia-Yi Yu, Shu-Yu Lin, Pin-Wei Shih, Pei-Yun Shu, Day-Yu Chao, Chao-Ying Pan, Hong-Ming Chen, Guey-Chuen Perng, Chia-Chi Ku*, and Chwan-Chuen King*. Use of seroprevalence to guide dengue vaccination plans for older adults in a dengue non-endemic country. PLOS Neglected Tropical Diseases. 2021. Accepted. (*correspondent author).
2. Hsin-Fang Tu, Chun-Jung Ko, Ching-Tai Lee, Cheng-Fan Lee, Shao-Wei Lan, Hsin-Hsien Lin, Hsin-Ying Lin, Chia-Chi Ku, Der-Yen Lee, I-Chun Chen, Ya-Hui Chuang, Francisco Del Caño-Ochoa, Santiago Ramón-Maiques, Geen-Dong Chang, Chao-Chi Ho, and Ming-Shyue Lee. Afatinib exerts immunomodulatory effects by targeting the pyrimidine biosynthesis enzyme CAD. Cancer Research, 2021. Accepted.
3. Lin YJ, Lee TL, and Chia-Chi Ku*.  Development of an economical DNA delivery system by “acufection” and its application to skin research. Journal of Visualized Experiments. 2017 February, doi:10.3791/55206; URL:http://www.jove/video/55206 (*correspondent author).
4. Jui-Hung Kao, Chaur-Dong Chen, Zheng-Rong Tiger Li, Ta-Chien Chan, Tsung-Hwa Tung, Yin-Hsia Chu, Hau-Yuan Cheng, Jien-Wei Liu, Fuh-Yuan Shih, Pei-Yun Shu, Chien-Chou Lin, Wu-Hsiung Tsai, Chia-Chi Ku*, Chi-Kung Ho*, Chwan-Chuen King*. The Critical Role of Early Dengue Surveillance and Limitations of Clinical Reporting – Implications for Non-Endemic Countries.  PLos One. 2016, AUG;11:e0160230. (* corresponding author)
5. Chia-Chi Ku*, Yi-Hsuan Chang, Yun Chien, Tsung-Lin Lee. Type I interferon inhibits varicella-zoster virus replication by interfering with the dynamic interaction between Mediator and IE62 within replication compartments. Cell & Bioscience, 2016, MAR; 6:212016. (* corresponding author)
6. Lee TL, Chang ML, Lin YJ, Tsai MH, Chang YH, Chuang CM, Chien Y, Sosinowski T, Wang CH, Chen YY, Lee CK, Chen JS, Wang LF, Kung* JT and Chia-Chi Ku*.  An alternatively spliced IL-15 isoform modulates abrasion-induced keratinocyte activation, 2015. Journal of Investigative Dermatology, 135: 1329-1337 (* corresponding author)
7. 7. Yen-Chun Chen, Cheing-Meei Liu, Jiiang-Huei Jeng, and Chia-Chi Ku*, 2014. Association of Pocket Epithelial Cell Proliferation in Periodontitis with TLR9 Expression and Inflammatory Response. Journal of the Formosan Medical Association. Association of Pocket Epithelial Cell Proliferation in Periodontitis with TLR9 Expression and Inflammatory Response. Journal of the Formosan Medical Association. Aug;113:549-556. http://dx.doi.org/10.1016/j.jfma.2012.07.043 (* correspondent author).
8. Adel M. Nour, Mike Reichelt, Chia-Chi Ku, Min-Yin Ho, Thomas C. Heineman, and Ann M. Arvin, 2011. Varicella-zoster virus infection triggers formation of the NLRP3 inflammasome complex.  J Biol Chem. 2011 May 20;286(20):17921-33. Epub 2011 Mar 8.
9. Chia-Chi Ku, Xi-Bing Che, Mike Reichelt, Jaya Rajamani, Anne Schaap-Nutt, Ke-Jung Huang, Marvin H. Sommer, Yi-Shun Chen, Yi-Yuan Chen, and Ann M. Arvin, 2011.   Herpes Simplex Virus-1 Induces Expression of a Novel MxA Isoform that Enhances Viral Replication.  Immunology and Cell Biology, 2011 Feb;89(2):173-82. Epub 2010 Jul 6.
10. Ann M. Arvin, Jennifer Moffat, Marvin Sommer, Stefan Oliver, Xibing Che, Leigh Zerboni and Chia-Chi Ku, 2010.  Varicella-Zoster Virus T cell Tropism and the Pathogenesis of Skin Infection, Current Topics in Microbiology and Immunology, 142: pp.189-209.

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