2025年江伯倫老師實驗室論文發表--免疫所碩士班陳恬萱
Particulate matter 2.5 aggravates airway inflammation by neutrophil-mediated inflammasome activation
Tien-Hsuan Chen1 , Charles C. K. Chou2, 3 ,Bor-Luen Chiang1, 4, 5*
- Graduate Institute of Clinical Medicine, National Taiwan University, Taipei, Taiwan.
- Air Quality Research Center, Academia Sinica, Taipei, Taiwan.
- Research Center for Environmental Changes, Academia Sinica, Taipei, Taiwan.
- Genome and Systems Biology Degree Program, College of Life Science, National Taiwan University, Taipei, Taiwan.
- Department of Pediatrics, National Taiwan University Hospital, Taipei, Taiwan.
Abstract
Background: Particulate matter 2.5 (PM2.5) aggravates allergic airway inflammation by inducing a type 2 helper T cell (Th2)-biased response and accumulation of oxidative stress. As detailed mechanisms about how they lower the threshold initiation of asthma are not fully understood, we postulated the role of neutrophilic inflammation and inflammasome activation in the effect. We evaluated the alternations of asthmatic indicators after intranasal PM2.5 exposure in adult BALB/c mice with ovalbumin (OVA) sensitization. PM2.5 exposure enhanced eosinophilic inflammation and induced neutrophilic inflammation in the lungs by generating both systemic and local Th2- and Th17-biased responses. PM2.5 enhanced nucleotide-binding domain leucine-rich repeat containing protein 3 (NLRP3) expression in the lungs and led to elevation of downstream IL-1β signaling. We identified neutrophils as the cellular source of PM2.5-enhanced inflammasome activation. Further experiments addressed that elevation of IL-1β signaling enhanced the chemokine production macrophage inflammatory proteins (MIP)-1α in neutrophils. Moreover, reduction of IL-1β signaling decreased lung infiltration in the disease model.These data suggested the contribution of neutrophil-mediated inflammasome activation in the PM2.5-induced adjuvant effect on airway allergic inflammation. The results provide a novel coping strategy for pollutant control and further the understanding of the etiology of asthma.
Keywords: allergic airway inflammation, asthma, inflammasome, neutrophil, particulate matter 2.5
Allergy. 2025 Apr;80(4):1176-1179