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2025年江伯倫老師實驗室論文發表--免疫所碩士班黃羿苹

2025-10-16最新消息

B Cells Induced Regulatory T Cells Attenuated the Classical M1 Polarization of Mouse Bone Marrow-Derived Macrophages

Yi-Ping Huang1, Chien-Hui Chien2, Li-Chieh Wang3, Bor-Luen Chiang1,3,4

  1. Graduate Institute of Immunology, National Taiwan University, Taipei, Taiwan
  2. Department of Medical Research, National Taiwan University Hospital Hsin-Chu Branch, Hsin-Chu County, Taiwan.
  3. Department of Pediatrics, National Taiwan University Hospital, Taipei, Taiwan
  4. Genome and Systems Biology Degree Program, College of Life Science, National Taiwan University, Taipei, Taiwan

Abstract

Regulatory T (Treg) cells are effective immunomodulators of adaptive and innate immune responses. Our previous studies have demonstrated that B-cell-induced CD4+Foxp3− regulatory T cells, referred to as Treg-of-B cells, exert suppressive capacity, inhibiting CD4+CD25- T-cell proliferation and inflammasome activation. In the present study, Treg-of-B cells downregulated proinflammatory M1-like markers and partially induced M2-like genes in unpolarized bone marrow-derived macrophages (BMDMs), as evidenced by RNA expression of Nos2, Arg1, Retnla, Mrc1, and Egr2. Treg-of-B cells decreased the RNA levels of Nos2, Tnfa, Cd86, and Cxcl9, and the production of tumor necrosis factor (TNF)-α, interleukin (IL)-6, and nitrite in LPS/interferon (IFN)-γ-stimulated M1-like macrophages in a dose-dependent manner. These cells also secreted Th2 cytokines, including IL-10, IL-4, and IL-13, with enhanced cytokine production observed upon cocultured with macrophages. Mechanistically, Treg-of-B cells exerted their modulatory effect via both cell-cell contact and contact-dependent induction of soluble mediators, particularly Th2 cytokines. Furthermore, Treg-of-B cells promoted IκBα accumulation and suppressed RNA expression of Kruppel-like factor 4 (Klf4), thereby inhibiting NF-κB activation. These findings suggest that Treg-of-B cells regulate macrophage plasticity and might prevent excessive inflammation.

Keywords: Treg-of-B cells, macrophages, IL-10, IL-4, nitrite, KLF4

Sci Rep 2025 October, 15(1): 35537

doi: 10.1038/s41598-025-19445-1

 

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